Nile Red is a lipophilic fluorescent dye that stains hydrophobic membranes and lipids. It can also be used as a non-specific stain for neutral lipids such as triacylglycerols and cholesterol esters. Nile Red has a characteristic bright red fluorescence that can be observed under an appropriate excitation wavelength. It is commonly used in microscopy, flow cytometry, and lipid analysis for the quantitation and visualization of lipid droplets in cells and tissues. Nile Red is also used in environmental and analytical chemistry for the detection and quantitation of hydrophobic pollutants and organic compounds in soil, water, and air samples. Overall, Nile Red is a versatile fluorescent dye with diverse applications in various fields of science and technology.
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